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this residue is hydrogen bonded on the lone drinking water molecule (or hydroxide) which is coordinated to your metallic ion bound to the Mβ-web site (21). As a result, it is expected this residue will perform in catalysis by abstraction of a proton from water ahead of, or concomitant with, the assault of hydroxide around the amide bond of your substrate. Inside the detection restrictions of our assay for item formation, we ended up struggling to evaluate any catalytic exercise for possibly the D273N or D273A mutant.
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